Monday, August 27, 2012

Stem Cell Research & Therapy | Abstract | Adipose stem cells can secrete angiogenic factors that inhibit hyaline cartilage regeneration

Stem Cell Research & Therapy | Abstract | Adipose stem cells can secrete angiogenic factors that inhibit hyaline cartilage regeneration

Adipose stem cells can secrete angiogenic factors that inhibit hyaline cartilage regeneration

Christopher SD Lee, Olivia A Burnsed, Vineeth Raghuram, Jonathan Kalisvaart, Barbara D Boyan and Zvi Schwartz

Stem Cell Research & Therapy 2012, 3:35 doi:10.1186/scrt126
Published: 24 August 2012

Abstract (provisional)

Introduction

Adipose stem cells (ASCs) secrete many trophic factors that can stimulate tissue repair, including angiogenic factors, but little is known about how ASCs and their secreted factors influence cartilage regeneration. Therefore, the aim of this study was to determine the effects ASC-secreted factors have in repairing chondral defects.

Methods

ASCs isolated from male Sprague Dawley rats were cultured in monolayer or alginate microbeads supplemented with growth (GM) or chondrogenic medium (CM). Subsequent co-culture, conditioned media, and in vivo cartilage defect studies were performed.

Results

ASC monolayers and microbeads cultured in CM had decreased FGF-2 gene expression and VEGF-A secretion compared to ASCs cultured in GM. Chondrocytes co-cultured with GM-cultured ASCs for 7 days had decreased mRNAs for col2, comp, and runx2. Chondrocytes treated for 12 or 24 hours with conditioned medium from GM-cultured ASCs had reduced sox9, acan, and col2 mRNAs; reduced proliferation and proteoglycan synthesis; and increased apoptosis. ASC-conditioned medium also increased endothelial cell tube lengthening whereas conditioned medium from CM-cultured ASCs had no effect. Treating ASCs with CM reduced or abolished these deleterious effects while adding a neutralizing antibody for VEGF-A eliminated ASC-conditioned medium induced chondrocyte apoptosis and restored proteoglycan synthesis. FGF-2 also mitigated the deleterious effects VEGF-A had on chondrocyte apoptosis and phenotype. When GM-grown ASC pellets were implanted in 1 mm non-critical hyaline cartilage defects in vivo, cartilage regeneration was inhibited as evaluated by radiographic and equilibrium partitioning of an ionic contrast agent via microCT imaging. Histology revealed that defects with GM-cultured ASCs had no tissue ingrowth from the edges of the defect whereas empty defects and defects with CM-grown ASCs had similar amounts of neocartilage formation.

Conclusions

ASCs must be treated to reduce the secretion of VEGF-A and other factors that inhibit cartilage regeneration, which can significantly influence how ASCs are used for repairing hyaline cartilage.

Friday, August 10, 2012

Urine Test Can Indicate A Woman's Risk Of Bone Fracture, Pitt Study Finds

Urine Test Can Indicate A Woman's Risk Of Bone Fracture, Pitt Study Finds

A simple urine test can indicate a premenopausal woman's risk of suffering bone fractures as she ages, according to new research led by University of Pittsburgh Graduate School of Public Health (GSPH) epidemiologists.

Women in their 40s and early 50s had a 59 percent greater risk of bone fracture as they aged when they had above-normal levels of N-telopeptide (NTX) - the byproduct of bones breaking down - in their urine, compared with women who had low NTX levels. When women with high NTX levels also had a low spinal bone density measurement, their risk of fracture increased nearly three-fold.

The study is the first to look for signs of bone breakdown in younger, premenopausal women in an effort to determine if such signs can predict the risk that these women will suffer fractures as they age.

The results were published today in the online edition of Menopause, the journal of The North American Menopause Society. The report will be published in the journal's November print issue.

"Bone fractures - particularly in the hip, wrist and back - have serious consequences, including disability and death," said Jane Cauley, Dr.P.H., professor of epidemiology, GSPH, and lead author of the study. "Knowing a woman's risk of fracture can help doctors determine the best course of action to protect her bones as she enters menopause, a time when estrogen deficiency negatively affects skeletal health."

By the time a woman turns 50, her risk of a fracture at some point in the remainder of her life is estimated to be at least 40 percent. Fractures are more common for these women than heart attacks, strokes and breast cancer combined.

During menopause, bone remodeling increases, leading to an imbalance between bone formation and bone resorption, or the process by which bones are broken down and their minerals are returned to the blood. This remodeling persists for several years and is associated with an increased rate of bone loss, making it easier for bones to fracture.

Cauley and her colleagues used data from 2,305 premenopausal or perimenopausal women aged 42 to 52 collected over an average of 7.6 years as part of the Study of Women's Health Across the Nation (SWAN). Participants were from Boston, Detroit, Los Angeles, Pittsburgh and Oakland, Calif.

SWAN examines the physical, biological, psychological and social health of women during their middle years. The goal is to help scientists, health care providers and women learn how mid-life experiences affect health and quality of life during aging.

Collaborators on this study include Michelle E. Danielson, Ph.D., Yue-Fang Chang, Ph.D., Kristine Ruppert, Dr.P.H., Leslie Meyn, M.S., and Beth A. Prairie, M.D., M.S., all of the University of Pittsburgh; Gail A. Greendale, M.D., and Carolyn J. Crandall, M.D., M.S., both of the University of California Los Angeles; Joel S. Finkelstein, M.D., and Robert M. Neer, M.D., both of Massachusetts General Hospital; Joan C. Lo, M.D., of Kaiser Permanente Northern California; and MaryFran R. Sowers, Ph.D., of the University of Michigan.

This research was supported by the National Institutes of Health (NIH), Department of Health and Human Services, through the National Institute on Aging, the National Institute of Nursing Research and the NIH Office of Research on Women's Health (grants NR004061, AG012495, AG012505, AG012531, AG012553 through AG012535, AG012539 and AG012546). This work also was supported by Department of Defense grant DAMD17-96-6118; NIH grants K24-DK02759 and RR-1066; the Iris Cantor-University of California, Los Angeles Women's Health Center; and University of California, Los Angeles Center of Excellence in Women's Health grant RFP 282-97-0025.  

Wednesday, August 8, 2012

Award-winning study details simple method to make anatomic ACL femoral tunnels | Orthopedics

Award-winning study details simple method to make anatomic ACL femoral tunnels | Orthopedics

Award-winning study details simple method to make anatomic ACL femoral tunnels

  • July 20, 2012
BALTIMORE — Research presented at the American Orthopaedic Society for Sports Medicine Annual Meeting 2012 yielded helpful guidelines for anatomic femoral tunnel placement in ACL reconstruction. 

“We know that it is important to produce an anatomic femoral tunnel and an anatomic ACL, but there are two questions,” Alexander D. Davis, MD, said in his presentation of the work, for which he and his colleagues received the Aircast Award for Basic Science. “How do we find the anatomic femoral insertion and how do we create a tunnel at this location?”
Davis and colleagues removed the medial condyles from 12 fresh, frozen distal femurs. They dissected all the soft tissue except the ACL attachment and marked the centers of the ACL and its two bundles. The researchers than observed the femur in 90° flexion and marked the lowest point on the lateral wall of the notch. This point was then used as the starting point for a vertical line, against which Davis and colleagues measured the height of the ACL center and its composite bundles. They then measured the distance from those center points to the front and back of the notch.
The team placed metal beads at these three centers and took lateral radiographs using the quadrant method, according to Davis. They then seated a 7-mm femoral offset aimer at the vertical height of the ACL center, he said, and placed a pin through the aimer to mark a point on the lateral wall of the notch.
Results of the study illustrated that the femoral attachment of the ACL and its bundles can be identified through a method based on the height of these structures on the lateral wall of the notch, Davis said. Intra-operatively, a 7-mm femoral offset aimer seated at the height of the central ACL directs a pin about halfway between the ACL central and anteromedial bundle attachments. Then the shallow and deep ACL positions and their bundles can be referenced to a vertical line that starts at the low point of the notch’s lateral wall or cartilage borders, he explained.
“Whether you are using rigid reamers in a hyperflexed position or flexible reamers, once you have established your starting point you can then produce an anatomic femoral tunnel that is based upon a reproducible reference point that can be seen intra-operatively based upon the lowest point of articular cartilage,” Davis said.
Reference:
Davis AD, Brown C, Steiner ME. Simple guidelines for anatomic femoral tunnel placement in ACL reconstruction. Paper #17. Presented at the American Orthopaedic Society for Sports Medicine Annual Meeting  2012. July 12-15. Baltimore.

Biomarkers could be used to prevent atrophy in ACL-deficient patients, study finds | Orthopedics

Biomarkers could be used to prevent atrophy in ACL-deficient patients, study finds | Orthopedics

Biomarkers could be used to prevent atrophy in ACL-deficient patients, study finds

Interesting paper and possible utility.

Pre-injury biomarkers linked with subsequent ACL injury | Orthopedics

Pre-injury biomarkers linked with subsequent ACL injury | Orthopedics

Pre-injury biomarkers linked with subsequent ACL injury

Very interesting topic from Westpoint that I saw at the AOSSM meeting in Baltimore this summer.

Tuesday, August 7, 2012

More Players Suffer Soccer Sprains When One Ankle Is Stronger

More Players Suffer Soccer Sprains When One Ankle Is Stronger

More Players Suffer Soccer Sprains When One Ankle Is Stronger

By Frederik Joelving

NEW YORK (Reuters Health) Jun 22 - Pro soccer players are much more likely to suffer ankle sprains when one foot is stronger than the other, Greek researchers have found.
There has been a slew of studies into the reasons athletes suffer sprains, but few have focused on soccer players, according to Dr. George Vagenas, of the University of Athens, and colleagues.
The researchers did preseason tests of ankle strength and stability in 100 players from four professional soccer teams in Greece. Then they followed the players over the next 10 months to see who would get hurt on the field.
Seventeen players suffered one or more non-contact sprains during the season. Those with considerable functional strength differences between their left and right ankles were nine times as likely to suffer sprains as those whose ankles were about the same strength.
When a player cuts or lands from a jump, Dr. Vagenas told Reuters Health by email, it's important to have symmetrical activation of the two sets of ankle muscles to help the joints absorb the impact and prevent damage.
He suggested that "all soccer players, professionals or not, must be evaluated during the preseasonal period by sports specialists for verification of potential functional asymmetry of the ankle joint."
But that might not be realistic, said Dr. Timothy A. McGuine, a sports medicine specialist at the University of Wisconsin-Madison.
"Most lay people won't have the time and money to do this kind of screening," he told Reuters Health.
But there is still an important message from the new study, even for amateur athletes, according to Dr. Erik Wikstrom, an expert in ankle sprains at The University of North Carolina at Charlotte.
"This study does suggest that if soccer players want to lower their risk of suffering ankle sprains, then they should strengthen their ankle musculature evenly, so that they have a good balance between both legs," Dr. Wikstrom told Reuters Health by email.
"This take-home message can apply to just about all athletes and non-athletes," he added. "Proper balance between the lower extremities is very important."
Earlier studies have shown that both lace-up ankle braces and balance training on a wobble board can help stave off injuries to the joint.
"I tell people to go ahead and balance on one leg, then the other for two to three minutes," said Dr. McGuine, who led those studies, adding that two to three times a week is a good start.
The Greek researchers, who published their study June 4 in the American Journal of Sports Medicine, also found that heavy players were more prone to sprains, which makes sense given the extra force their ankles have to absorb when they land or cut.
Apart from keeping a healthy weight, Dr. McGuine told Reuters Health that it's important for athletes to learn how to land properly, too.
"Don't land stiff legged," he said. "We want a soft foot strike."
SOURCE: http://bit.ly/MoUQb5
Am J Sports Med, 2012.

'Double-Jointed' Soccer Players Have More Injuries

'Double-Jointed' Soccer Players Have More Injuries

'Double-Jointed' Soccer Players Have More Injuries

By Amy Norton
NEW YORK (Reuters Health) Jan 03 - Soccer players with hypermobile joints may have a higher injury risk than their less flexible teammates, a study of one professional team suggests.
Benign joint hypermobility syndrome is diagnosed when a person is found to have at least four abnormally flexible joints -- based on tests of whether knees or elbows can bend backwards, the thumb can be flexed to touch the forearm, the pinkie finger can bend backward beyond 90 degrees and they can place their palms on the floor without bending the knees.
Hypermobile joints are not as stable as less-flexible joints, so in theory they could be more vulnerable to injuries like sprains. But researchers have come to conflicting conclusions on whether hypermobile athletes do sustain more injuries.
For the new study, UK researchers followed 54 men on an English Premier League soccer team over one season.
Of the players, 18 (one third of the group) were deemed hypermobile. And over the season, those 18 men suffered 72 injuries -- for a rate of 22 injuries for every 1,000 hours of practice and competition.
By comparison, the 36 players with less-flexible joints sustained 61 injuries: a rate of just over six injuries per 1,000 hours.
The mean difference in injury rates, 15.65 injuries/1000 h, was significant at p=0.001.
Matt D. Konopinski and colleagues at Leeds Metropolitan University reported the findings online December 16 in the American Journal of Sports Medicine.
The study adds to evidence that general hypermobility contributes to sports injuries, according to Verity Pacey, a physical therapist at the Children's Hospital in Westmead, Australia, who has studied the question.
In a recent study, Pacey and her colleagues found that across contact sports, players with joint hypermobility were nearly five times more likely to suffer a knee injury than their less-flexible counterparts.
That was based on a meta-analysis that combined the results of 18 previous studies.
It's not known yet whether weekend athletes with extra-flexible joints face the same risks as professional athletes seem to, Pacey told Reuters Health in an email.
Amateur athletes do not go through the amount of intense training and competition that pros do, pointed out Gareth J. Jones, one of the researchers on the current study.
On one hand, that might protect the hypermobile weekend athlete, Jones told Reuters Health in an email.
"However," he added, "they are also generally less well conditioned, which may increase the risk."
In this study, soccer players' injuries were often relatively mild -- like muscle strains, cramps or tears in the legs.
But some injuries were severe, meaning they kept players out of the game for at least 28 days. And hypermobile players were much more likely to have a severe injury.
Twelve of the 18 athletes suffered at least one severe injury during the season -- often a ligament or cartilage tear in the knee. That compared with only two of the 36 non-hypermobile athletes.
The knee is especially vulnerable to injury in soccer, Jones said. And for people with hypermobile joints, ligaments and other structures in the knee may be "less able to cope" with the stress placed on them.
Exercises to boost strength, muscle control and balance can help hypermobile people who already have joint pain or injuries.
And it's possible that such training could curb their risk of future sports injuries, Jones said.
But whether that is the case is not clear.
"Unfortunately," Pacey said, "at present we don't have any strong research evidence to support ways we can reduce the risk of injury in hypermobile sporting participants."
In general, researchers still aren't sure exactly how harmful hypermobile joints might be. Some extra-flexible people have chronic joint pain, but many don't. And there's no evidence yet that people with hypermobile joints face an increased arthritis risk.
One issue is that studies have varied widely in estimating how common hypermobility is in the general public, or among athletes.
A recent study, though, found that among teenagers, hypermobility is common -- a sign, the researchers said, that such flexibility is often perfectly normal.
Of 6,000 teenagers the researchers assessed, 27% of girls and 11% of boys met the criteria for benign joint hypermobility syndrome.
The current findings suggest that hypermobility could be very common in soccer, according to Konopinski's team. But it's not clear, they add, whether it's any more common in soccer than in other sports, or compared with the public at large.
Pacey said there is research underway to better understand hypermobility, its effects and, when needed, how to manage it.
One unknown, Pacey noted, is why some people who are hypermobile in their youth become less flexible as they age. "We've yet to understand why this occurs in only some individuals."
SOURCE: http://bit.ly/uICyg8
Am J Sports Med 2011.